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serum free t4 levels  (Monobind)


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    Structured Review

    Monobind serum free t4 levels
    TRIAC effects on the hypothalamus-pituitary-thyroid axis in hypothyroid mice (A–D) Serum levels of 3,3′,5,5′-tetraiodothyronine <t>(T4),</t> 3,3′,5-triiodothyronine (T3), TRIAC, and thyroid-stimulating <t>hormone</t> <t>(TSH).</t> (E) Gross appearance of thyroid glands. The scale on the ruler is 1 mm. (F) Thyroid gland size was measured as thyroid area on photograph. (G–J) Histological images of thyroid glands stained using hematoxylin and eosin. Vehicle, PTU, PTU + TRIAC, n = 6 each; PTU + LT3, n = 5. BLQ, below the limit of quantitation. Concentrations of PTU, LT3, and TRIAC used were 100 μg/mL, 0.1 μg/mL, and 0.1 μg/mL, respectively. Data are represented as means ± SEM. Statistical analyses were performed using Student’s t -test for vehicle vs. PTU and the p value is presented as †††p < 0.001. ANOVA followed by the Tukey-Kramer test was used for comparisons among PTU, PTU + LT3, and PTU + TRIAC experiments and the p value is presented as ∗∗p < 0.01, and ∗∗∗p < 0.001.
    Serum Free T4 Levels, supplied by Monobind, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/serum free t4 levels/product/Monobind
    Average 96 stars, based on 3 article reviews
    serum free t4 levels - by Bioz Stars, 2026-03
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    1) Product Images from "TRIAC disrupts cerebral thyroid hormone action via negative feedback and heterogenous distribution among organs"

    Article Title: TRIAC disrupts cerebral thyroid hormone action via negative feedback and heterogenous distribution among organs

    Journal: iScience

    doi: 10.1016/j.isci.2023.107135

    TRIAC effects on the hypothalamus-pituitary-thyroid axis in hypothyroid mice (A–D) Serum levels of 3,3′,5,5′-tetraiodothyronine (T4), 3,3′,5-triiodothyronine (T3), TRIAC, and thyroid-stimulating hormone (TSH). (E) Gross appearance of thyroid glands. The scale on the ruler is 1 mm. (F) Thyroid gland size was measured as thyroid area on photograph. (G–J) Histological images of thyroid glands stained using hematoxylin and eosin. Vehicle, PTU, PTU + TRIAC, n = 6 each; PTU + LT3, n = 5. BLQ, below the limit of quantitation. Concentrations of PTU, LT3, and TRIAC used were 100 μg/mL, 0.1 μg/mL, and 0.1 μg/mL, respectively. Data are represented as means ± SEM. Statistical analyses were performed using Student’s t -test for vehicle vs. PTU and the p value is presented as †††p < 0.001. ANOVA followed by the Tukey-Kramer test was used for comparisons among PTU, PTU + LT3, and PTU + TRIAC experiments and the p value is presented as ∗∗p < 0.01, and ∗∗∗p < 0.001.
    Figure Legend Snippet: TRIAC effects on the hypothalamus-pituitary-thyroid axis in hypothyroid mice (A–D) Serum levels of 3,3′,5,5′-tetraiodothyronine (T4), 3,3′,5-triiodothyronine (T3), TRIAC, and thyroid-stimulating hormone (TSH). (E) Gross appearance of thyroid glands. The scale on the ruler is 1 mm. (F) Thyroid gland size was measured as thyroid area on photograph. (G–J) Histological images of thyroid glands stained using hematoxylin and eosin. Vehicle, PTU, PTU + TRIAC, n = 6 each; PTU + LT3, n = 5. BLQ, below the limit of quantitation. Concentrations of PTU, LT3, and TRIAC used were 100 μg/mL, 0.1 μg/mL, and 0.1 μg/mL, respectively. Data are represented as means ± SEM. Statistical analyses were performed using Student’s t -test for vehicle vs. PTU and the p value is presented as †††p < 0.001. ANOVA followed by the Tukey-Kramer test was used for comparisons among PTU, PTU + LT3, and PTU + TRIAC experiments and the p value is presented as ∗∗p < 0.01, and ∗∗∗p < 0.001.

    Techniques Used: Staining, Quantitation Assay



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    TRIAC effects on the hypothalamus-pituitary-thyroid axis in hypothyroid mice (A–D) Serum levels of 3,3′,5,5′-tetraiodothyronine (T4), 3,3′,5-triiodothyronine (T3), TRIAC, and thyroid-stimulating hormone (TSH). (E) Gross appearance of thyroid glands. The scale on the ruler is 1 mm. (F) Thyroid gland size was measured as thyroid area on photograph. (G–J) Histological images of thyroid glands stained using hematoxylin and eosin. Vehicle, PTU, PTU + TRIAC, n = 6 each; PTU + LT3, n = 5. BLQ, below the limit of quantitation. Concentrations of PTU, LT3, and TRIAC used were 100 μg/mL, 0.1 μg/mL, and 0.1 μg/mL, respectively. Data are represented as means ± SEM. Statistical analyses were performed using Student’s t -test for vehicle vs. PTU and the p value is presented as †††p < 0.001. ANOVA followed by the Tukey-Kramer test was used for comparisons among PTU, PTU + LT3, and PTU + TRIAC experiments and the p value is presented as ∗∗p < 0.01, and ∗∗∗p < 0.001.

    Journal: iScience

    Article Title: TRIAC disrupts cerebral thyroid hormone action via negative feedback and heterogenous distribution among organs

    doi: 10.1016/j.isci.2023.107135

    Figure Lengend Snippet: TRIAC effects on the hypothalamus-pituitary-thyroid axis in hypothyroid mice (A–D) Serum levels of 3,3′,5,5′-tetraiodothyronine (T4), 3,3′,5-triiodothyronine (T3), TRIAC, and thyroid-stimulating hormone (TSH). (E) Gross appearance of thyroid glands. The scale on the ruler is 1 mm. (F) Thyroid gland size was measured as thyroid area on photograph. (G–J) Histological images of thyroid glands stained using hematoxylin and eosin. Vehicle, PTU, PTU + TRIAC, n = 6 each; PTU + LT3, n = 5. BLQ, below the limit of quantitation. Concentrations of PTU, LT3, and TRIAC used were 100 μg/mL, 0.1 μg/mL, and 0.1 μg/mL, respectively. Data are represented as means ± SEM. Statistical analyses were performed using Student’s t -test for vehicle vs. PTU and the p value is presented as †††p < 0.001. ANOVA followed by the Tukey-Kramer test was used for comparisons among PTU, PTU + LT3, and PTU + TRIAC experiments and the p value is presented as ∗∗p < 0.01, and ∗∗∗p < 0.001.

    Article Snippet: We measured serum free T4 levels using an FT3, FT4, TSH AccuLite VAST CLIA kit (Monobind Inc., Lake Forest, CA) with Spark (Tecan, Zurich, Switzerland).

    Techniques: Staining, Quantitation Assay